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Figure 3 | Virology Journal

Figure 3

From: The interferon-inducible antiviral protein Daxx is not essential for interferon-mediated protection against avian sarcoma virus

Figure 3

Daxx is induced by type I IFNs. (A) Immunoblot analysis of Daxx protein levels (antibody from Sigma) after human (h) IFN-α (1000 U/ml) treatment of HeLa cells for the indicated time points. (B) Real-time PCR showing Daxx mRNA levels in DF-1 cells following treatment with chicken (ch) IFN-α for the indicated time points. Values were normalized to GAPDH. (C) Immunoblot analysis of Daxx protein levels following chicken (ch) IFN-α treatment of DF-1 cells for the indicated times. β-actin (antibody from Sigma) is shown as a loading control. Molecular-weight markers, in kilodaltons, are shown to the left of each blot.

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Virology Journal

ISSN: 1743-422X