Figure 1

Type I IFNs inhibit ASV replication. (A) Fluorescence-activated Cell Sorter (FACS) analysis of ASV-GFP replication (indicated by % GFP-positive cells) in untreated, human IFN-α (1000 U/ml)- or human IFN-β (1000 U/ml)-treated HeLa cells 48 h post-infection from a representative experiment. GFP fluorescence data were collected on an LSR II flow cytometer (Becton Dickinson), and analyzed using FlowJo software. FSC = Forward scatter. (B) Quantification of GFP-positive cells from four independent replicates of the experiment described in panel A. Error bars represent mean +/- standard deviation. * p <0.05. (C) VSV-GFP replication (indicated by % GFP-positive cells) in untreated, IFN-α (1000 U/ml)- or IFN-β (1000 U/ml)-treated HeLa cells 24 h post-infection from a representative experiment. (D) Quantification of GFP-positive cells from four independent replicates of the experiment described in panel C. Error bars represent mean +/- standard deviation. ***p <0.001.