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Table 3 Real-time quantitative RT-PCR analysis of the inhibition of FMDV replication in pEGFP-miR242 + 276-transformed BHK-21 cells, compared with normal BHK-21 cells

From: Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo

FMDV

Ct values (mean)

12 h.p.i.

24 h.p.i.

36 h.p.i.

48 h.p.i.

60 h.p.i.

72 h.p.i.

O/Tibet/China/1/99

34.61 ± 0.32/37.73 ± 0.07

36.20 ± 0.09/38.76 ± 0.25

35.98 ± 0.83/36.09 ± 0.12

37.55 ± 0.22/36.75 ± 0.44

37.62 ± 0.51/26.92 ± 0.21

36.08 ± 0.08/28.49 ± 0.39

O/HN/CHA/93

36.45 ± 0.05/35.65 ± 0.66

36.85 ± 0.57/31.32 ± 0.10

33.41 ± 0.15/28.62 ± 0.40

28.54 ± 0.10/28.82 ± 0.49

26.84 ± 0.16/26.34 ± 0.11

26.34 ± 0.30/26.54 ± 0.22

O/CHN/Mya98/33-P

16.70 ± 0.10/16.27 ± 0.23

15.17 ± 0.02/14.57 ± 0.04

11.81 ± 0.30/12.44 ± 0.14

8.43 ± 0.59/14.16 ± 0.41

7.49 ± 0.31/9.16 ± 0.04

13.68 ± 0.10/15.02 ± 0.59

AF72

30.97 ± 0.08/25.23 ± 0.17

36.23 ± 0.78/30.44 ± 0.41

36.36 ± 0.18/35.02 ± 0.79

38.45 ± 1.07/25.21 ± 0.92

36.32 ± 0.94/10.32 ± 0.71

37.13 ± 0.15/11.16 ± 0.31

Asia1/Jiangsu/China/2005

36.73 ± 0.18/34.16 ± 0.68

37.12 ± 0.11/35.65 ± 0.19

37.28 ± 0.39/35.46 ± 0.04

35.42 ± 0.09/27.39 ± 0.20

36.62 ± 0.57/17.84 ± 0.63

38.59 ± 0.77/16.68 ± 0.44

  1. Viral supernatants were collected at 12, 24, 36, 48, 60, and 72 h.p.i., and the Ct values were derived from two parallel experiments (treatment/control).
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Virology Journal

ISSN: 1743-422X