Skip to main content

Table 2 Efficiencies of miRNAs targeting the FMDV IRES in inhibiting EGFP expression in BHK-21 cells as assayed by flow cytometry

From: Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo

Reporter plasmid

Inhibition efficiency of each miRNA (%)

pmiR153

pmiR220

pmiR242

pmiR276

Bi-miRNA

Dual-miRNA

pHN/IRES-EGFP

72.2%

56.7%

44.3%

81.4%

84.7%

95.0%

pFC/IRES-EGFP

38.7%

47.9%

71.4%

60.5%

88.3%

96.6%

pJS/IRES-EGFP

64.6%

37.5%

68.8%

62.5%

78.4%

83.6%

  1. Bi-miRNA, a mixture of pmiR242 and pmiR276 plasmids; Dual-miRNA, a co-cistronic expression plasmid (pmiR242 + 276) containing two pre-miRNA (pmiR242 and pmiR276) hairpin structures. Percentage inhibition in each co-transfected (vector-delivered miRNAs and the reporter plasmids) cell population was calculated by comparison with the control cells transfected only with the same reporter plasmid at 48 h post-transfection.
Back to article page

Virology Journal

ISSN: 1743-422X