Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo

Table 1 Oligonucleotides of vector-delivered pre-miRNAs

Name	Single stranded DNA sequences (5′ → 3′)		Position
pmiR153	Top strand	TGCTGCTCCTTGGTAACAAGGACCCAGTTTTGGCCACTGACTGACTGGGTCCTTTACCAAGGAG	151–171
pmiR153	Bottom strand	CCTGCTCCTTGGTAAAGGACCCAGTCAGTCAGTGGCCAAAACTGGGTCCTTGTTACCAAGGAG C	151–171
pmiR220	Top strand	TGCTGGCACGGCAACTTTACTGTGAAGTTTTGGCCACTGACTGACTTCACAGTAGTTGCCGTGC	220–240
pmiR220	Bottom strand	CCTGGCACGGCAACTACTGTGAAGTCAGTCAGTGGCCAAAACTTCACAGTAAAGTTGCCGTGC C	220–240
pmiR242	Top strand	TGCTGCCACCTTAAGGTGACACTGATGTTTTGGCCACTGACTGACATCAGTGTCCTTAAGGTGG	243–263
pmiR242	Bottom strand	CCTGCCACCTTAAGGACACTGATGTCAGTCAGTGGCCAAAACATCAGTGTCACCTTAAGGTGG C	243–263
pmiR276	Top strand	TGCTGCACTGGTGACAGGCTAAGGATGTTTTGGCCACTGACTGACATCCTTAGTGTCACCAGTG	277–297
pmiR276	Bottom strand	CCTGCACTGGTGACACTAAGGATGTCAGTCAGTGGCCAAAACATCCTTAGCCTGTCACCAGTG C	277–297
pmiR-NC	Top strand	TGCTGAAATGTACTGCGCGTGGAGACGTTTTGGCCACTGACTGACGTCTCCACGCAGTACATTT	Heterologous
pmiR-NC	Bottom strand	CCTGAAATGTACTGCGTGGAGACGTCAGTCAGTGGCCAAAACGTCTCCACGCGCAGTACATTT C	Heterologous

The sense sequences of predicted miRNAs targeting the FMDV IRES are shown in bold and italic.

ISSN: 1743-422X