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Figure 3 | Virology Journal

Figure 3

From: Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo

Figure 3

PCR analysis and morphological observation of the FMDV IRES-specific Dual-miRNA-transformed cell clones. Cultured cells were transfected with pEGFP-miR242 + 276 (BHK-21 cells) or pmiR242 + 276 (goat fibroblasts), and the resultant stably transformed clones were obtained by BlasticidinR selection as described in Methods. (A) The PCR products from harvested cells were electrophoresed through 0.8% agarose gels and identified under UV light. As a negative control, a mixture of the total DNA extracted from normal BHK-21 cells and goat fibroblasts was amplified using the same specific primer pairs. (B) The green fluorescence in the pEGFP-miR242 + 276-transformed BHK-21 cells (left) and the pmiR242 + 276-transformed goat fibroblasts (right) were visualized with a fluorescence microscope and a light microscope, respectively.

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