Table 4 Real-time quantitative RT-PCR primers
RNA target | Forward primer (5’ – 3’) | Reverse primer (5’ – 3’) | Genbank accession # | Ref. |
|---|---|---|---|---|
IFNA | GACAGCCAACGCCAAAGC | AATGCTTGAGCAGCAGCGAC | EF053034 | (5) |
IFNB | CCTCAACCAGATCCAGCATT | GGATGAGGCTGTGAGAGGAG | AY831397 | (5) |
IFNG | CAACGCTCAACTACTCTC | TGTGGTTAATCTGTCCTTAG | AJ012254 | DK |
IL1B | TCGACATCAACCAGAAGTGC | GAGCTTGTAGCCCTTGATGC | DQ393268 | (5) |
IL2 | GCCAAGAGCTGACCAACTTC | ATCGCCCACACTAAGAGCAT | AF294323 | (5) |
IL6 | TTCGACGAGGAGAAATGCTT | CCTTATCGTCGTTGCCAGAT | AB191038 | (5) |
MHCI | GAAGGAAGAGACTTCATTGCCTTGG | CTCTCCTCTCCAGTACGTCCTTCC | AB115246 | (5) |
MHCII | CCACCTTTACCAGCTTCGAG | CCGTTCTTCATCCAGGTGAT | AY905539 | (5) |
TLR7 | CCTTTCCCAGAGAGCATTCA | TCAAGAAATATCAAGATAATCACATCA | AY940195 | (5) |
GAPDH | ATGTTCGTGATGGGTGTGAA | CTGTCTTCGTGTGTGGCTGT | AY436595 | (5) |