Western blot of VP6 protein . Empty vector-transforming bacterial protein (negative control) and VP6-bearing bacterial protein were transferred onto a nitrocellulose membrane, then, the membrane was consecutively incubated with the anti-VP6 antibody and HRP-conjugated secondary antibody. Their blot results are shown in lanes 2 and 3, respectively. Lane 1: protein marker. The size of protein marker and the VP6 protein is shown.