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Figure 5 | Virology Journal

Figure 5

From: Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses

Figure 5

Optimization of dilution of anti-VP6 antibody in ELISA. Purified VP6 protein (5 μg/well) was diluted and coated in ELISA wells, and the serially diluted anti-VP6 antibody was used as primary antibody in subsequent ELISA. Dilutions of anti-VP6 antibody are indicated. The OD490 value of tested samples (P)/the OD490 value of negative control, coating buffer (N)>2 is judged as positive and shown as a curve; the broken-line is trend line.

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