Bacterial expression of VP6 protein in E.coli. Expression of VP6 protein was detected by a Coomassie blue staining. PAGE gel screened with rabbit antiserum to the VP6 as the primary antibody. Lane 1, crude bacterial lysate from cells containing non-recombinant vector only; Lane 2, non-induced crude bacterial lysate from bacteria containing VP6 plasmid; Lane 3, protein marker; Lane 4 unlysed VP6-containing bacteria at 4 h post-induction; Lane 5, lysed VP6-containing bacterial supernatant at 4 h post-induction; Lane 6, lysed VP6-containing bacterial inclusion bodies. The size of protein marker and the VP6 protein is indicated.