Detection of TBEV gene from mouse tissue samples by TBEV RT-LAMP assay. Visual detection of RT-LAMP products using 10 ng of RNA samples extracted from blood, plasma, liver, spleen and brain samples collected from laboratory mice on the 5th and 9th day following peripheral infections with TBEV Oshima strain. Three mice in each group and one mouse for mock infected mice were used. Positive amplification was indicated by the change of the color to cloudy yellow (A) or fluorescent green under UV irradiation (B). The reaction was repeated three times for each sample and a representative result is shown.