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Figure 2 | Virology Journal

Figure 2

From: Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification

Figure 2

Gene amplification and detection of different TBEV subtypes by subtype-specific RT-LAMP assay. Real-time kinetics was monitored by the turbidity of RT-LAMP products after using 10 ng of RNA samples extracted from supernatants of cultured cells infected with the Oshima (FE), Sofjin (FE), IR-99 (Sib) and Hochostervitz (Eu) strains and JEV JaOArS982 strain. Each viral gene was amplified by (A) TBEV-specific RT-LAMP assay, (B) TBEV FE subtype-specific RT-LAMP assay, (C) TBEV Sib subtype-specific RT-LAMP assay, or (D) TBEV EU subtype-specific RT-LAMP assay. Each reaction was repeated three times and a representative result is shown for each sample. (E) Real-time kinetics of TaqMan rRT-PCR with TBEV-specific primer/probe set using 10 ng of RNA samples extracted from supernatants of cultured cells infected with the Oshima (FE), Sofjin (FE), IR-99 (Sib) and Hochostervitz (Eu) strains and JEV JaOArS982 strain. The reaction was repeated three times and a representative result is shown.

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Virology Journal

ISSN: 1743-422X