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Figure 1 | Virology Journal

Figure 1

From: Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification

Figure 1

Gene amplification and detection of the TBEV by TBEV-specific RT-LAMP assay. (A) Real-time kinetics of the RT-LAMP amplification was monitored by real-time turbidimeter. In vitro transcribed RNA of TBEV Oshima strain was serially diluted to make 10-1 to 104 copies. The reaction was repeated three times and a representative result is shown. (B) Agarose gel electrophoresis profile of the RT-LAMP products. M indicates 100-bp DNA marker (Sigma). N indicates a sample containing no viral RNA. (C and D) Visual detection of the RT-LAMP products amplified in a reaction tube. Positive amplification is indicated by change of color to cloudy yellow (C) or by fluorescent green under UV irradiation (D). (E) Real-time kinetics of TaqMan rRT-PCR with TBEV-specific primer/probe set. In vitro transcribed RNA of Oshima was serially diluted to make 10-1 to 106 copies. The reaction was repeated three times and a representative result is shown.

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Virology Journal

ISSN: 1743-422X