Figure 8
Immunofluoresent staining of virus infected suckling mouse brain sections. a) Two day old suckling mice were infected intranasaly with 105 pfu of either T1L or T3D before fluorescent antibody staining of frozen coronal brain sections at 7 days pi. T1L and T3D antigens were stained with virus specific antiserum as described in Figure 5. Cell nuclei were stained with DAPI, shown in blue. Size bars are 100 μM. b) Horse Radish Peroxidase staining (in brown) of formalin fixed paraffin embedded brains of T1L and T3D infected suckling mouse brains at 7 days pi. Staining of T3D infected brains showed the presence of neurons and neuronal processes whereas the cell type infected by T1L were non-neuronal and thus lacked processes. Staining of endothelial cells in blood vessels was also observed for both T3D (not shown ) as well as T1L infected brain that is shown in insert. Images were collected with the 20X objective lens and size bars are indicated.