Figure 7
Time course of immunofluorescent staining of virus infected suckling lung. Two day old suckling mice were infected intranasaly with 105 pfu of either T1L or T3D before fluorescent antibody staining of frozen lung sections after 1, 2, or 3 days of infection. T1L and T3D antigens were stained with virus specific antiserum as described in Figure 5. Cell nuclei were stained with DAPI, shown in blue. Size bars are 100 μM. Sagital sections show viral infection patterns similar to those of adults ( Figures 5 and 6) that again show sparse staining of epithelium in the bronchiolar airways (arrow) being restricted primarily to T3D infection.