|
Experiments
|
Primer names
|
Primer sites (5′-3′)
|
Primer sequences (5′-3′)
|
|---|
|
cDNA library screening
|
P1-F
|
37664-37645
|
ACAGCGAGCAGCAGGAGTT
|
| |
P1-R
|
37365-37384
|
AGAGCCCGTCGTGATAGTCC
|
| |
P2-F
|
38763-38744
|
ACCGCCTGCGACTGCCGCAT
|
| |
P2-R
|
38564-38583
|
TACAACAACACGCAGGGCTG
|
|
Northern blotting a and RT-PCR
|
P0-F
|
37250-37231
|
TAGTCTCGTTTTTTATTAAA
|
| |
P0-R
|
37011-37030
|
CAGTCCGCGACGATCCACAG
|
| |
P1-Fb
|
37664-37645
|
ACAGCGAGCAGCAGGAGTT
|
| |
P1-Rb
|
37365-37384
|
AGAGCCCGTCGTGATAGTCC
|
| |
P2-Fb
|
38763-38744
|
ACCGCCTGCGACTGCCGCAT
|
| |
P2-Rb
|
38564-38583
|
TACAACAACACGCAGGGCTG
|
| |
P3-F
|
40306-40288
|
CGTCGGTGTTCCTTCCTT
|
| |
P3-R
|
39997-40014
|
CATGCCCGTCGTGCTCTT
|
| |
P4-F
|
42945-42926
|
GTCAACTTTCTGCGCCATCT
|
| |
P4-R
|
42498-42516
|
GCTGCACCTCCGTATCCTT
|
| |
P3-1-F
|
40673-40654
|
AGAAACCGGTGCTGGGCAAG
|
| |
P3-1-R
|
40430-40447
|
ACGGACGCCGAGGCTGAC
|
| |
P3-2-F
|
41023-41005
|
TCCCTTCAGGATGCCTACG
|
| |
P3-2-R
|
40784-40801
|
TCGGACGACGGTGTTGTG
|
| |
P3-3-F
|
41361-41344
|
GATCCGCGTTTCACCGAC
|
| |
P3-3-R
|
41115-41132
|
CCCAGGGCGAGTTACCGT
|
|
3′ RACE
|
GSP-3’out primer
|
37713- 37694
|
CATGTAGCCGACTTGGAGGA
|
| |
P1-F
|
37664-37645
|
ACAGCGAGCAGCAGGAGTT
|
| |
P2-R
|
38564 -38583
|
TACAACAACACGCAGGGCTG
|
| |
UL31-3’-in
|
39310-39327
|
CCGCAACCCGTCACTCTT
|
|
5′ RACE
|
GSP-5’out primer
|
37342- 37359
|
AAAGGCACGCTGTTGACG
|
| |
P1-R
|
37365-37384
|
AGAGCCCGTCGTGATAGTCC
|
| |
GSP-5’-2
|
37801-37820
|
CGGGAAGAGGTTCTTCTCCC
|
| |
GSP-5’-3
|
38349-38367
|
ACGTGGTGACCTCGTGGAT
|
| |
GSP-5’-4
|
38743-38762
|
CATGCGGCAGTCGCAGGCGG
|
| |
GSP-5’-5
|
42522-42539
|
GCACAAAGGCGATGGGTT
|
| |
GSP-5’-6
|
42803-42823
|
CGGTAGTATCCCAACCAAAGC
|
- a. The additional oligonucleotide of 5′-AATACGACTCACTATAGG-3′ was added to the 5′ ends of the reverse primers for the templates of all the Northern blotting probes and acted as the promoter of T7 RNA polymeras.
- b. These primers were for the template of the complementary probes of probe-1 and anti-probe-1, as well as probe-2 and anti-probe-2, which were switched as the forward and reverse primers.
