Analysis and mapping of a 3′ coterminal transcription unit derived from human cytomegalovirus open reading frames UL30–UL32

Table 1 The primers used in the present study

Experiments	Primer names	Primer sites (5^′-3^′)	Primer sequences (5^′-3^′)
cDNA library screening	P₁-F	37664-37645	ACAGCGAGCAGCAGGAGTT
	P₁-R	37365-37384	AGAGCCCGTCGTGATAGTCC
	P₂-F	38763-38744	ACCGCCTGCGACTGCCGCAT
	P₂-R	38564-38583	TACAACAACACGCAGGGCTG
Northern blotting ^a and RT-PCR	P₀-F	37250-37231	TAGTCTCGTTTTTTATTAAA
	P₀-R	37011-37030	CAGTCCGCGACGATCCACAG
	P₁-F^b	37664-37645	ACAGCGAGCAGCAGGAGTT
	P₁-R^b	37365-37384	AGAGCCCGTCGTGATAGTCC
	P₂-F^b	38763-38744	ACCGCCTGCGACTGCCGCAT
	P₂-R^b	38564-38583	TACAACAACACGCAGGGCTG
	P₃-F	40306-40288	CGTCGGTGTTCCTTCCTT
	P₃-R	39997-40014	CATGCCCGTCGTGCTCTT
	P₄-F	42945-42926	GTCAACTTTCTGCGCCATCT
	P₄-R	42498-42516	GCTGCACCTCCGTATCCTT
	P_3-1-F	40673-40654	AGAAACCGGTGCTGGGCAAG
	P_3-1-R	40430-40447	ACGGACGCCGAGGCTGAC
	P_3-2-F	41023-41005	TCCCTTCAGGATGCCTACG
	P_3-2-R	40784-40801	TCGGACGACGGTGTTGTG
	P_3-3-F	41361-41344	GATCCGCGTTTCACCGAC
	P_3-3-R	41115-41132	CCCAGGGCGAGTTACCGT
3^′ RACE	GSP-3’out primer	37713- 37694	CATGTAGCCGACTTGGAGGA
	P₁-F	37664-37645	ACAGCGAGCAGCAGGAGTT
	P₂-R	38564 -38583	TACAACAACACGCAGGGCTG
	UL31-3’-in	39310-39327	CCGCAACCCGTCACTCTT
5^′ RACE	GSP-5’out primer	37342- 37359	AAAGGCACGCTGTTGACG
	P₁-R	37365-37384	AGAGCCCGTCGTGATAGTCC
	GSP-5’-2	37801-37820	CGGGAAGAGGTTCTTCTCCC
	GSP-5’-3	38349-38367	ACGTGGTGACCTCGTGGAT
	GSP-5’-4	38743-38762	CATGCGGCAGTCGCAGGCGG
	GSP-5’-5	42522-42539	GCACAAAGGCGATGGGTT
	GSP-5’-6	42803-42823	CGGTAGTATCCCAACCAAAGC

a. The additional oligonucleotide of 5^′-AATACGACTCACTATAGG-3^′ was added to the 5^′ ends of the reverse primers for the templates of all the Northern blotting probes and acted as the promoter of T7 RNA polymeras.
b. These primers were for the template of the complementary probes of probe-1 and anti-probe-1, as well as probe-2 and anti-probe-2, which were switched as the forward and reverse primers.

ISSN: 1743-422X