5′RACE results of the transcripts from the UL31anti-UL32 transcription Unit using the L class RNA of HCMV infected HELF cells as the sample. A. 5′ RACE results of the 0.6 and 6.0 kb transcripts using primers of P1-R and GSP5′-6 as the gene specific 5′ RACE primer, respectively. All the controls of TAP (−) and M-MLV (−) were negative, except for the TAP (−) control when amplified with primer P1-R. The product of the TAP (−) control was not consistent with those of the sample, and was not sequenced. B. The 5′ RACE results of the transcripts between 1049 and 1821 bp using primers of GSP5′-2, GSP5′-3 and GSP5′-4 as the gene specific primer, respectively. After amplification with primer GSP 5′-2, a product similar to the 750 bp product of the sample was found in the TAP (−) control. However, sequencing result showed that the 5′ end was not consistent with that of the 750 bp product of the sample.