Figure 5

5^′RACE results of the transcripts from the UL31anti-UL32 transcription Unit using the L class RNA of HCMV infected HELF cells as the sample. A. 5^′ RACE results of the 0.6 and 6.0 kb transcripts using primers of P1-R and GSP5^′-6 as the gene specific 5^′ RACE primer, respectively. All the controls of TAP (−) and M-MLV (−) were negative, except for the TAP (−) control when amplified with primer P1-R. The product of the TAP (−) control was not consistent with those of the sample, and was not sequenced. B. The 5^′ RACE results of the transcripts between 1049 and 1821 bp using primers of GSP5^′-2, GSP5^′-3 and GSP5^′-4 as the gene specific primer, respectively. After amplification with primer GSP 5^′-2, a product similar to the 750 bp product of the sample was found in the TAP (−) control. However, sequencing result showed that the 5^′ end was not consistent with that of the 750 bp product of the sample.