Figure 4

Confocal microscopy analysis of 1-48preS/S, G2A 1-48preS/S and G2S 1-48preS/S proteins. BHK-21 cells were infected with rSFV encoding the respective proteins as described in Materials and Methods section and stained with MAb 1-9C1 (α-S), followed by polyvalent rabbit antibodies H863 (α-preS1). The cell nuclei were stained with DAPI. Panel A depicts images of cells, panel B shows the fluorescence intensity of each fluorophore - FITC, TRITC and DAPI (y axis, relative numbers) along a line (x axis, μm) drawn across a selected cell which is marked by the white arrow.