Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus-infected cells reveals virus-specific regulation of immune response genes

Table 1 Induction of EGF family members and genes involved in regulation of MAPK activity by CPXV, MPXV and VACV

	Gene	Gene expression changes (average FC)^a
	Gene	CPXV	MPXV	VACV
MAPK activity	DUSP5	6.6	4.9	3.6
	DUSP6	8.1	9.5	5.2
	EGR1	14.3	30.8	98.6
	EGR2	2.6	5.1	4.2
	IL4R	6.2	5.8	2.9
	LIF	9.7	12.6	4.6
	SPRY1	12.7	n.s.^b	n.s.^b
	SPRY2	7.7	7.1	7.0
	SPRY4	20.8	30.1	11.8
	SPRED1	2.8/3.7^c	2.9/3.0^c	2.2/2.2^c
	SPRED2	2.0	2.3	2.8/7.8^c
both	CBLB	−2.7	−3.4	−2.5
ErbB pathway	AREG	10.2	8.3	4.8
	EREG	4.4/6.7^c	7.0/10.3^c	2.9/3.0^c
	HBEGF	4.0/5.4^c	2.3/2.6^c	2.1/2.8^c
	PRKCB	−4.9	−3.4	−2.4

^a Cluster and pathway analysis of the 87 non-histone transcripts that were affected by all OPV tested revealed a statistically significant overrepresentation of genes involved in inactivation of mitogen-activated protein kinase (MAPK) activity and growth factor signalling. Shown are gene expression changes of the identified genes at 6 h post infection in CPXV-, VACV- or MPXV-infected cells in comparison to mock-infected cells as average fold change (FC) values.
^b Values that did not meet the analysis cut-off of an average FC ≥2.0 and p ≤0.05 are designated n.s. (not significant).
^c Two values separated by a slash designate values obtained from two different probes on the microarray.

ISSN: 1743-422X