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Table 1 Intra and inter-assay variations in PPRNT

From: Pseudo-plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus

Virus Input

Intra-assay variation

Inter-assay variation

 

Ratio between duplicate test in same serum

*Six sera with known FFRNT

 

<4

4-7

7-10

Total

32 (n=2)

128 (n=2)

512 (n=2)

 

n (%)

n (%)

n (%)

n

**GMT(CV)

GMT(CV)

GMT(CV)

50 PPFU/well

20 (28.98)

33 (47.85)

16 (23.18)

69

36.76 (4.63)

111.4 (7.70)

445.70 (10.81)

     

27.86 (9.19)

147.0 (4.79)

588.10 (7.53)

  1. *Neutralizing anti-CCHF antibodies titers were directly assigned to the highest dilution with > 50% reduction.
  2. **GMT; geometric mean titer, CV; coefficient of variance = (S.D./mean) × 100%, which was calculated with logarithmic transformation.
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Virology Journal

ISSN: 1743-422X