Primer & Probe | Sequence | Concentration | Orientationa | Positionb | Tm (°C) |
---|---|---|---|---|---|
Flavi all S | TACAACATgATggggAARAgAgARAA | 10 pmol | S | 8993–9019 | 54.7 |
DEN4 F | TACAACATgATgggRAAACgTgAGAA | 10 pmol | S | 8996–9019 | 60.18 |
Flavi all AS 1 | gTCCCANCCDgCKgTRTC | 10 pmol | AS | 9236–9253 | 52.5 |
 | gTCCCATCCAgCKgTRTCATC | 5 pmol | AS | 9236–9256 | 57.1 |
Flavi all AS 2 | gTgTCCCAgCCNgCKgTgTCATCWgC | 10 pmol | AS | 9232–9260 | 69.6 |
Flavi all probe 1 | FAM-AARggHAgYMgNgCCA + T H + T + g + g + T-BBQ | 5 pmol | S | 9044–9065 | 69–83 |
Flavi all probe 2 | FAM-Tg + g TWYATgTggYTNg + g RgC--BBQ | 5 pmol | S | 9062–9082 | 62–75 |
Flavi all probe 3 mix | FAM-Tg + g TWYATgT + g gYTNg + g RgC—BBQc | 5 pmol | S | 9062–9082 | 66-79 |
 | FAM-CCgTgCCATATggTATATgTggCTgggAgC-BBQd | 0.5 pmol | S | 9052–9081 | 74.7 |
 | FAM-TTTCTggAATTTgAAgCCCTgggTTT-BBQe | 0.5 pmol | S | 9086–9012 | 68 |
Flavi all S2 | TACAACATgATgggMAAACgYgARAA | 10 pmol | S | 8996–9019 | 58.2 |
Flavi all AS4 | gTgTCCCAGCCNgCKgTRTCRTC | 10 pmol | S | 9235–9260 | 64.1 |