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Table 4 Test of sensitivity of Pan-Flavi assay with pre-quantified viral RNA dilutions

From: Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses

Quantity (Genome copies/reaction) Ct values for DENV-1 Ct values for TBEV strain K23 Ct values for YFV strain 17D
1 × 107 16.8 16.6 -
1 × 106 20.5 19.5 20.8
1 × 105 23.4 23.0 24.2
1 × 104 27.4 26.2 27.6
1 × 103 30.2 29.4 30.1
1 × 102 37.1 31.7 36.4
1 × 101 39.7 33.0 No Ct
  1. GC: genome copies, Ct: threshold cycle.
  2. YFV: Yellow fever virus, TBE: Tick-borne encephalitis virus, DENV: Dengue virus.