Virus | Strain/ Acc. No. | Pan-flavi assay*,# | Virus-specific assay# | |||||
---|---|---|---|---|---|---|---|---|
Mean of Ct value | SD of Ct value | Mean of GC/ml | Mean of Ct value | SD of Ct value | Mean of GC/ml | Reference | ||
Flavivirus | ||||||||
DENV-1 | ATCC VR-344 | 15.64 | 0.02 | 2.0 × 109 | 15.93 | 0.03 | 4.6 × 109 | [29] |
DENV-2 | ATCC VR-345 | 20.76 | 0.20 | 6.6 × 107 | 18.77 | 0.11 | 6.7 × 108 |  |
DENV-3 | ATCC VR-1256 | 19.36 | 0.01 | 1.7 × 108 | 20.30 | 0.01 | 2.4 × 108 |  |
DENV-4 | ATCC VR-1257 | 16.33 | 0.19 | 1.3 × 109 | 16.18 | 0.29 | 3.9 × 109 |  |
YFV 17D | X03700 | 19.17 | 0.17 | 1.9 × 108 | 20.51 | 0.17 | 5.6 × 108 | [30] |
YFV ASIBI | RKI reference strain | 23.73 | 0.05 | 9.1 × 106 | 23.72 | 0.28 | 4.7 × 107 |  |
YFV Brazil | RKI reference strain | 21.18 | 0.07 | 5.0 × 107 | 22.50 | 0.10 | 1.2 × 108 |  |
YFV Ivory Cost | RKI reference strain | 21.44 | 0.21 | 4.2 × 107 | 21.29 | 0.05 | 3.1 × 108 |  |
WNV Uganda | AY532665, lineage 1 | 28.74 | 0.06 | 3.2 × 105 | 26.83 | 0.27 | 3.4 × 106 | [31] |
WNV Israel | lineage 2 | 19.81 | 0.15 | 1.2 × 108 | 19.86 | 0.01 | 4.0 × 108 |  |
TBEV K23 | AF091010 | 16.33 | 0.03 | 1.3 × 109 | 16.78 | 0.14 | 2.1 × 108 | [32] |
RSSEV | RKI reference strain | 24.20 | 0.32 | 6.7 × 106 | 19.35 | 0.01 | 4.1 × 106 |  |
TBEV Louping ill | RKI reference strain | 20.59 | 0.22 | 7.4 × 107 | 20.43 | 0.06 | 2.0 × 107 |  |
JEV | ATCC SA14-14-2 | 19.36 | 0.01 | 2.4 × 108 | 16.73 | 0.01 | 7.7 × 108 | In house assay |