Table 1 List of primers used for the amplification of the HCV coding region
RT primer | Oligo d(A) | 5’-AAAAAAAAAAAAAAAAAAAA-3’ | |
|---|---|---|---|
PCR1 primers | Subtype 1a | GEN1.F1: | 5’-CAAGACTGCTAGCCGAGTAGTGTTGGGTCG-3’ |
|  |  | GEN1A.R1 | 5’-CCGGGCAYGAGACACGCTGTGATAAATG-3’ |
|  | Subtype 1b | GEN1.F1 | 5’-CAAGACTGCTAGCCGAGTAGTGTTGGGTCG-3’ |
|  |  | GEN1B.R1 | 5’-TCGGGCACGAGACAVGCTGTGATATATG-3’ |
PCR2 primers | Subtype 1a | GEN1.F2 | 5’-GTACTGCCTGATAGGGTGCTTGCGAGTGCC-3’ |
|  |  | GEN1A.R2 | 5’-TCTCCCCCGCTGTAGCCAGCCGTGAACC-3’ |
|  | Subtype 1b | GEN1.F2 | 5’-GTACTGCCTGATAGGGTGCTTGCGAGTGCC-3’ |
|  |  | GEN1B.R2 | 5’-TCTCCCCCGCTGTARCCAGCRACGAACC-3’ |