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Table 1 List of primers used for the amplification of the HCV coding region

From: Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples

RT primer Oligo d(A) 5’-AAAAAAAAAAAAAAAAAAAA-3’
PCR1 primers Subtype 1a GEN1.F1: 5’-CAAGACTGCTAGCCGAGTAGTGTTGGGTCG-3’
   GEN1A.R1 5’-CCGGGCAYGAGACACGCTGTGATAAATG-3’
  Subtype 1b GEN1.F1 5’-CAAGACTGCTAGCCGAGTAGTGTTGGGTCG-3’
   GEN1B.R1 5’-TCGGGCACGAGACAVGCTGTGATATATG-3’
PCR2 primers Subtype 1a GEN1.F2 5’-GTACTGCCTGATAGGGTGCTTGCGAGTGCC-3’
   GEN1A.R2 5’-TCTCCCCCGCTGTAGCCAGCCGTGAACC-3’
  Subtype 1b GEN1.F2 5’-GTACTGCCTGATAGGGTGCTTGCGAGTGCC-3’
   GEN1B.R2 5’-TCTCCCCCGCTGTARCCAGCRACGAACC-3’