Figure 3

Down-regulation of IL-32 expression by hcmv-miR-UL112-1. (A) The diagram shows the predicted sequences of hcmv-miR-UL112-1 binding to IL-32 mRNA. (B) As a candidate target, IL-32 was validated for its ability to inhibit expression of a luciferase reporter construct in the presence of hcmv-miR-UL112-1 (pS-miR-UL112-1). Results are shown as percentage expression of negative control sample (pS-neg) following correction for transfection levels according to the control of renilla luciferase expression. Values are shown as means ± standard deviations for triplicate samples. (C) HEK 293 cells were transfected with pS-miR-UL112-1 or control vector respectively. Cells were collected 48 hpi and were subjected to western blot analysis using the indicated antibodies. IL-32 densitometer values were normalized to that of the β-actin values. (D) The amounts of proteins presented in panel C were quantified by densitometry. Results are shown as percentage expression of the negative control sample (Empty).