Expression of IL-32 induced by HCMV infection in MRC-5 cells. (A) IL-32 mRNA levels in HCMV infected cells were compared to that in uninfected MRC-5 cells. β-actin was used as an internal control. The universal primers for all IL-32 transcripts were used. U is for uninfected MRC-5; IE is for immediate early stage; E is for early stage; L is for late stage. (B) IL-32 mRNA levels were represented relative to that in IE stage. (C) IL-32 protein levels in HCMV infected MRC-5 cells were detected at different time points. IL-32 densitometer values were normalized by β-actin values. (D) The IL-32 protein levels were represented relative to that in infected cells collected at 6 hpi. (E) The kinetics of hcmv-miR-UL112-1 expression were measured using TaqMan® miRNA assays. The expression of hcmv-miR-UL112-1 could be detected at 24 hpi and increased gradually as the HCMV-infection process was prolonged.