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Figure 2 | Virology Journal

Figure 2

From: Lineage shift in Indian strains of Dengue virus serotype-3 (Genotype III), evidenced by detection of lineage IV strains in clinical cases from Kerala

Figure 2

Immunofluorescence analysis of DENV-3 infected C6/36 mosquito cell line. Cells grown in 24-well cell culture plates were infected with the indicated virus strains for three days. The viral replication was detected by immunostaining using anti-dengue virus polyclonal serum as primary antibody and goat anti-rabbit IgG Alexa Fluor® 488 conjugated antibody as the secondary antibody. Uninfected cells and cells treated only with the secondary antibody are shown as controls. The cytoplasmic fluorescent foci in the infected cells indicate the sites of virus replication. Blue staining regions indicate the nuclei of the cells counterstained with DAPI . Scale bar represents 50 μm.

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Virology Journal

ISSN: 1743-422X