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Table 3 qPCR confirmation of differentially expressed genes

From: Transcriptome analysis of primary monocytes from HIV-positive patients with differential responses to antiretroviral therapy

Gene

Comparison

qPCR

Microarray

Gene

Comparison

qPCR

Microarray

ACTN4

VIRvsCTR

3.1

3.6

IL1B

VIRvsCTR

-12.5

-13.2

ACTN4

VIRvsBDL

1.8

2.3

IL1B

BDLvsCTR

-17.0

-20

ITGAL

VIRvsCTR

1.9

2.4

CSK

VIRvsCTR

3.7

2.9

ITGAL

VIRvsBDL

2.3

2.5

CFL1

VIRvsCTR

2.5

2.2

GNAI2

VIRvsCTR

2.7

2.9

CD37

VIRvsCTR

2.3

2.4

SERPING1

VIRvsCTR

3.8

2.6

C1QB

VIRvsBDL

2.6

2.3

IL8

VIRvsCTR

-4.6

-5.0

C1QB

VIRvsCTR

4.1

4.3

  1. Fold change by qPCR was obtained from the mean expressions of the tested genes in each group. The cohort for qPCR validation consisted of 10 viremic patients, 10 BDLs, and 9 healthy controls (including all the original samples used in the microarray). All values represent fold changes between expression levels of the first group versus expression levels of the second group. Minus sign indicates down-regulation in the first group whereas positive sign indicates up-regulation in the first group. Housekeeping gene GAPDH was used as an internal control and the normalizer in qPCR.