Gene | Comparison | qPCR | Microarray | Gene | Comparison | qPCR | Microarray |
---|
ACTN4 | VIRvsCTR | 3.1 | 3.6 | IL1B | VIRvsCTR | -12.5 | -13.2 |
ACTN4 | VIRvsBDL | 1.8 | 2.3 | IL1B | BDLvsCTR | -17.0 | -20 |
ITGAL | VIRvsCTR | 1.9 | 2.4 | CSK | VIRvsCTR | 3.7 | 2.9 |
ITGAL | VIRvsBDL | 2.3 | 2.5 | CFL1 | VIRvsCTR | 2.5 | 2.2 |
GNAI2 | VIRvsCTR | 2.7 | 2.9 | CD37 | VIRvsCTR | 2.3 | 2.4 |
SERPING1 | VIRvsCTR | 3.8 | 2.6 | C1QB | VIRvsBDL | 2.6 | 2.3 |
IL8 | VIRvsCTR | -4.6 | -5.0 | C1QB | VIRvsCTR | 4.1 | 4.3 |
- Fold change by qPCR was obtained from the mean expressions of the tested genes in each group. The cohort for qPCR validation consisted of 10 viremic patients, 10 BDLs, and 9 healthy controls (including all the original samples used in the microarray). All values represent fold changes between expression levels of the first group versus expression levels of the second group. Minus sign indicates down-regulation in the first group whereas positive sign indicates up-regulation in the first group. Housekeeping gene GAPDH was used as an internal control and the normalizer in qPCR.