Figure 3From: Characterization of an envelope gene VP19 from Singapore grouper iridovirusSubcellular localization of VP19 in transfected cells. After transfection with different plasmids, including pEGFP-C1 and EGFP-VP19, cells were fixed and the immune fluorescence assay were carried out using anti-VP19 primary antibody and Alexa Fluor® 555 Conjugated anti-mouse IgG. The green fluorescence indicated the distribution of EGFP-VP19 fusion protein, and the red fluorescence showed the VP19 protein recognized by antibody against VP19.Back to article page