Figure 1

Schematic rVSV NiV _B vaccine genomes and groups. (A) Group 1: rVSV-ΔG-GFP expressing no glycoprotein; produces ‘bald’ virions, Group 2 G_Ind*rVSV-NiV_B /F-GFP expressing the NiV_B F protein (blue); produces virions with F on the surface, Group 3 G_Ind*rVSV-NiV_B /G-GFP expressing the NiV_B G protein (yellow); produces virions with G on the surface, Group 4 made from co-infection in Vero cells resulting in a combination of rVSV-NiV_B /F-GFP and rVSV-NiV_B /G-GFP virions expressing the NiV_B F and G proteins in the same cells; produces single-cycle infectious virions with NiV_B F and G proteins on the cell surface (green). (B) Phase and fluorescence microscopy of Vero cells infected with virions from Group 4 displaying multinucleated syncytia cells * and cells positive for GFP expression.