Figure 3
From: Molecular investigation of the 7.2 kb RNA of murine cytomegalovirus
The m106 open reading frame is translated during MCMV infection. (A) Diagram of the GFP cassette insertion within the MCMV Smith BAC clone. (B) Analysis of recombinant virus replication in mouse fibroblasts. Cells were infected at a multiplicity of 0.05 PFU/cell and viral supernatants were collected daily and titrated. Graph represents two biological replicates. (C) Western blot analysis of protein lysates prepared from mock-, MCMV:m106GFP (WT-GFP), or MCMVdel SD:m106GFP (SDM-GFP) infected cells at 48 h p.i. m106-GFP expression was detected using a polyclonal anti-GFP antibody. Asterisk denotes m106-GFP. (D) Mouse fibroblasts were infected with MCMV:m106-GFP (MOI = 0.05). Cells were fixed at 72 h p.i. and m106-GFP protein expression and 7.2 kb intron production was detected by by combined immunofluorescence assay and FISH.