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Table 2 Sequences of primers and PCR conditions

From: Detection of hepatitis C virus (HCV) negative strand RNA and NS3 protein in peripheral blood mononuclear cells (PBMC): CD3+, CD14+ and CD19+

  Sequences of primers PCR conditions
Reverse transcription 5′-TGR TGC ACG GTC TAC GAG ACC TC-3′ Temp. 72°C/30 min
I PCR 5′-RAYCACTCCCCTGTGAGGAAC-3′ 1 min at 94°C;
50 cycles of 94°C for 15 s, 58°C for 30 s, and 72°C for 30 s; 72°C for 7 min
II PCR 5′-ACTGTCTTCACGCAGAAAGCGTC-3′ 1 min at 94°C;
5′-CAAGCACCCTATCAGGCAGTACC-3′ 30 cycles of 94°C for 15 s, 58°C for 1 min, and 72°C for 1 min; at 72°C for 7 min