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Figure 4 | Virology Journal

Figure 4

From: Transcription factor regulation and cytokine expression following in vitro infection of primary chicken cell culture with low pathogenic avian influenza virus

Figure 4

C-Jun, p50 and IRF-3 activity in CEH in response to LPAIVs. Cells were infected for different time periods with LPAIVs at MOI of 1 and supplemented with 1 μg/ml trypsin or without trypsin in the medium. Cell lysates (10μg/ml) were tested for binding of the activated c-Jun, IRF-3 and p50 subunits using the Trans-Am ELISA kit. The results are expressed as specific binding (absorbance measured in the presence of the mutated oligonucleotides minus that measured in the presence of the wild-type oligonucleotides) according to the manufacturer’s instructions and are compared to non-infected control.

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Virology Journal

ISSN: 1743-422X