Detection of BARF-1 protein by Western blotting. Immunoblotting of BARF-1 on seven cervical tumor biopsies. Fifty micrograms of protein extract as measured by a Biorad protein assay were deposited onto 12% polyacrylamide gel and electrophoresed. Proteins were transferred onto nitrocellulose paper. BARF1 protein encoding 29 kDa (p29) was revealed by PepIII rabbit polyclonal anti-BARF-1. BARF-1 protein (p29) produced by the BARF-1 recombinant adenovirus system was used as a positive control indicated in first lane as BARF1.