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Figure 4 | Virology Journal

Figure 4

From: Interleukin-1β receptor expressed by modified vaccinia virus Ankara interferes with interleukin-1β activity produced in various virus-infected antigen-presenting cells

Figure 4

MVAΔIL-1βR increases IL-1β biological activity after infection of human monocytic cells. THP-1 or THP-1 shASC (down-regulated inflammasome) cells were pre-stimulated with 7.8 ng/ml PMA for 8 hours and subsequently infected with the indicated viruses at five MOI. 16 hours after infection, cell-free supernatants were transferred onto HEK-Blue IL-1β reporter cells, which respond to human IL-1β by expressing a reporter gene (SEAP). HEK-Blue IL-1β reporter cells were then incubated overnight and induced SEAP levels were measured by spectrophotometry. The intensity of the color reaction is proportional to the amount of IL-1β transferred from the culture supernatant of the infected THP-1 cells. THP-1 cells treated with 1 μg/ml LPS served as a positive control. IL-1β activity transferred from infected THP-1 cells is given relative to the amount of IL-1β produced in uninfected THP-1 cells (=1). Means of two independent infections per cell type and virus are shown with standard deviations. Data were analyzed with an unpaired t-test using Graph Pad Prism software. (* = p < 0.05).

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Virology Journal

ISSN: 1743-422X