Figure 3

MVAΔIL-1βR induces IL-1β production in activated mouse peritoneal macrophages. C57BL/6 mice were immunized intraperitoneally with 10⁸ pfu MVAwt, MVAΔIL-1βR, or MVA-IL-1βRrev, or mock-treated with PBS. Six days after infection, peritoneal exudate cells (PEC) were collected by washing the abdominal cavity with 5–7 ml of PBS. A Isolated PEC from each immunized group of mice (n = 2) were pooled and cultured in medium. One day after cultivation, adherent cells (macrophages) were infected in duplicate with MVAwt or MVAΔIL-1βR at five MOI, or mock infected for 24 hours. Means of the two infections per virus and immunization group are shown with standard deviations. Data were evaluated by a mixed linear model for repeated procedures (mouse) with fixed factor immunization group (mock, MVAwt, MVAΔIL-1βR, and MVA-IL-1βRrev). Statistical analyses were performed with SAS®/STAT software, version 9.3, SAS System for Windows. (* = p-value < 0.05 and ** = p-value < 0.01). B Three mice/group were immunized with the different viruses as described above. Isolated PEC from each mouse were stained for F4/80, CD11b, and CD11c as macrophage lineage markers and for CD80 and MHC class II as activation markers. Percentages of MHC class II positive (left panel) or CD80 positive (right panel) F4/80⁺, CD11b⁺, and CD11c^medium/low PEC from each mouse are represented by one dot. Means of the three mice per group are shown.