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Table 2 Primers for cloning CMV-launched pKUN-∆-NS1

From: Non-structural protein-1 is required for West Nile virus replication complex formation and viral RNA synthesis

Primer

Sequence (5′ – 3′) ( MluI)

NS1-Mlu1-A-forward

GCTGATACTGGATGTACGCGTGATATAAGTCGGCAAG

NS1-Mlu1-A-reverse

CTTGCCGACTTATATCACGCGTACATCCAGTATCAGC

NS1-Mlu1-B-forward

CATCGTGGACCTGCCACGCGTACCACTACAGAGAG

NS1-Mlu1-B-reverse

CTCTCTGTAGTGGTACGCGTGGCAGGTCCACGATG

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Virology Journal

ISSN: 1743-422X