Table 1 Primers for cloning NS1 from different flaviviruses
Primers | Sequence (5′-3′) |
|---|---|
WNV NS1 forward | GTGGATGGGCGGCCGCACCATGGATAGGTCCATAGCTCTCACGTTT |
WNV NS1 reverse | CATTGACTGCGGCCGCTAAGCATTCACTTGTGACTGCAC |
DENV NS1 forward | GCGGCCGCACCATGGTCTCACTGTCTGTGACACTAG |
DENV NS1 reverse | TCAAGCTGTGACCAAGGAGTTGAC |
YFV NS1 forward | GCGGCCGCACCATGGACATGACAATGTCCATGAGCA |
YFV NS1 reverse | TCAAGCTGTAACCCAGGAGCGCACCAG |
JEV NS1 forward | GCGGCCGCACCATGGACCGATCAATTGCTTTGGCC |
JEV NS1 reverse | TCAAGCATGAACCTGTGATCTGACG |
SLEV NS1 forward | GCGGCCGCACCATGGACAGGAGCATCTCGCTGACTC |
SLEV NS1 reverse | TCAAGCTGTCACGCGAGATTTCACAAG |