Figure 6

Antiviral state is short-lived and virus non-specific. (A) The inner chamber bystander cells were harvested and challenged with MuV at an MOI of 0.1. Whole cell lysates were harvested after 48 hours and viral titers were measured by TCID₅₀. (B) To assess the duration of the anti-viral state, U937 cells were suspended in TW inner-chamber conditioned media and incubated for the indicated time at 37°C then challenged with MuV at an MOI of 0.1. Whole cell lysates were harvested after 48 hours and viral titers were measured by TCID₅₀. (C) The inner chamber bystander cells were harvested and challenged with MuV at an MOI of 0.1. Whole cell lysates were harvested after 48 hours and viral titers were measured by TCID₅₀. Data presented are representative of two-three experiments performed in triplicate (N = 2-3). *p < 0.05, **p < 0.01.