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Figure 5 | Virology Journal

Figure 5

From: In vitro inhibition of mumps virus by retinoids

Figure 5

Retinoids induce an anti-MuV state in the uninfected, bystander cells. U937 cells were infected with MuV at an MOI of 0.01 in the presence of 1 μM ATRA or DMSO. Transwell membrane inserts with 0.02 μm pores were used to separate the infected cells in the outer chamber from the uninfected, bystander cells in the inner chamber [48]. Cells from control wells (no membrane insert), outer and inner chamber bystander cells were harvested after 48 hours and IRF-1 (A) and RIG-I (B) mRNA were measured by qPCR. As indicated on the Figure, outer chamber cells infected by MuV are represented by open bars and inner chamber (uninfected) cells are represented by the filled bars. (C) Conditioned media from the control and transwell inner chambers were applied to fresh U937 cells. After 24 hours of incubation with the conditioned media, RNA was extracted and RIG-I expression was analyzed. Data presented reflect three experiments performed in triplicate (N = 3). * p <.02,**p < 0.01.

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