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Figure 4 | Virology Journal

Figure 4

From: In vitro inhibition of mumps virus by retinoids

Figure 4

RIG-I is required for the inhibition of MuV by retinoids. (A) Huh7 and Huh7.5 cells were infected with MuV at an MOI of 0.01 and treated with 1 μM ATRA or DMSO. Whole cell lysates were harvested after 48 hours and viral titers were measured by TCID50. (B) Huh7.5 cells were transfected with mock, pcDNA3.1 or pRIG-I-myc and incubated overnight. Following transfection, the cells were infected with MuV at an MOI of 0.01 and treated with 1 μM ATRA. Whole cell lysates were harvested after 48 hours and viral titers were measured by TCID50. Western blotting was not performed since Huh7.5 cells produce a defective RIG-I protein that cannot be distinguished from the wild-type protein by commercially-available antibodies. Data presented reflect two experiments performed in triplicate (N = 2). ***p < 0.001.

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