Hepatocyte ultrastructure in persistent HBV infection of tree shrews under TEM. A. Mitochondria were commonly swollen and enlarged, matrix electron density increased, and cristae were vague. M, mitochondria; N, nuclei. Osmic acid staining, 20000×. B. Electron dense rhabdoid crystalline inclusions (arrow) in mitochondria. Osmic acid staining, 30000×. C. Endoplasmic reticulum expanded or formed irregular vesicles with slight degranulation, and the expanded segments were surrounded by paired membranes, forming concentric lamellar bodies. R, reticulum. Osmic acid staining, 60000×. D. Suspected HBV granules in endoplasmic reticulum (arrow). Osmic acid staining, 60000×. E. The number of lysosomes (arrow) increased. Osmic acid staining, 20000×. F. Cell nuclei appeared irregular in shape and chromatin margination was observed. Osmic acid staining, 20000×.