Figure 3

Lack of HTLV-2 amplification. Representative nested PCR amplification of 17 samples (10 patient and 7 healthy controls) (lanes 1–10 and 11–17 respectively) visualized on 2% agarose real-safe stained gels. Panel A shows the products of the first round of PCR amplification (outer primers), while Panel B shows the nested PCR final products (inner primers). Lane 18 corresponds to a HTLV-2 patient positive gDNA kindly provided by Drs. Treviño and Soriano [26], and lane 19 is a negative control with no DNA template. Panel C shows amplification of the gag HTLV-2 gene from the pGEMT-HTLV-2-gag construct (see Methods) (number of copies 0–1000 as indicated) spiked into 1 μg of human gDNA with the inner amplification primers and conditions described in Methods. All PCR products were visualized on 2% agarose real-safe stained gels. M: molecular DNA markers correspond to 100 bp ladder marker (Promega) (panels A and B) and PCR markers (Promega) (panel C).