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Figure 2 | Virology Journal

Figure 2

From: Lack of evidence for retroviral infections formerly related to chronic fatigue in Spanish Fibromyalgia patients

Figure 2

Amplified products with gag primers were unspecific. Representative nested PCR amplification of 17 gDNA samples from 9 patient samples (lanes 1–9) and 8 healthy controls (lanes 10–17) visualized on 2% agarose real-safe stained gels. Lane 18 corresponds to the VP62 XMRV positive control and lane 19 is a negative control with no DNA template. M is the 100 bp ladder marker (Promega). Panel A shows the products amplified with the GAG-I-F and GAG-I-R primers (410 bp) [7, 44] while Panel B shows the products of the NP116 and NP117 primers (380 bp) [8]. All amplifications were performed under the permissive conditions described in materials and methods.

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Virology Journal

ISSN: 1743-422X