Skip to main content
Figure 4 | Virology Journal

Figure 4

From: Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry

Figure 4

Chemical inhibition of virus internalization pathways. BHK-21 cells were pretreated with (right panel) or without (left panel) NA alone or together with inhibitor 10 μM IPA3 or 10 mM MβCD or a mixture of the two chemicals at 37°C for 1 h. The cells were washed and infected with 5 × 102 TCID50 of rLa-egg, rLa-EBOVGP-egg, rLa-cell/TPCK rLa-EBOVGP-cell, or rLa-EBOVGP-cell/TPCK at 4°C for 1 h in the presence of the chemical(s). The cells were then washed and incubated at 37°C. At 7 h after infection, the cells were washed, fixed, permeabilized, and immunostained with chicken anti-NDV antiserum, then incubated with a FITC-conjugated rabbit anti-chicken antibody.

Back to article page

Virology Journal

ISSN: 1743-422X