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Figure 3 | Virology Journal

Figure 3

From: Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry

Figure 3

Neutralization assay. rLa-cell/TPCK, rLa-EBOVGP-cell, rLa-EBOVGP-cell/TPCK, rLa-egg, and rLa-EBOVGP-egg (each 5 × 102 TCID50) were mixed individually with chicken anti-NDV antiserum (diluted 1:100), mouse anti-EBOV GP antiserum (diluted 1:10), or a mixture of chicken and mouse antisera at the same dilutions. The viruses were also mixed with a combination of SPF chicken serum and naïve mouse serum as the mock-treated groups. After incubation, the viruses were allowed to infect BHK-21 cells for 1 h. The cells were fixed 48 h after infection, treated with chicken anti-NDV antiserum, and then incubated with a FITC-conjugated rabbit anti-chicken antibody.

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