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Figure 1 | Virology Journal

Figure 1

From: Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry

Figure 1

Generation of recombinant NDV expressing the EBOV GP gene. (A) Schematic representation of the rLa genome, showing the restriction site for endonuclease PmeI, introduced between the P and M genes, and the EBOV GP gene inserted into the PmeI site. (B) Indirect immunofluorescent staining of rLa-EBOVGP-infected BHK-21 cells with chicken anti-NDV antiserum and mouse anti-EBOV GP antiserum, observed with confocal laser microscopy. (C) Western blotting of sucrose-gradient-purified rLa and rLa-EBOVGP, hybridized with chicken anti-NDV antiserum and mouse anti-EBOV GP antiserum.

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Virology Journal

ISSN: 1743-422X