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Table 5 Primers used in this study

From: Molecular epidemiology of Newcastle disease virus isolates from vaccinated commercial poultry farms in non-epidemic areas of Japan

Primer name Nucleotide positiona Primer sequence
NDV-For1 1-15 5′-GGCCACGCGTCGACTAGTACACCAAACAGAGAATC-3′b
NDV-For482 482-501 5′-TYTGAGGAGAGRGCACAGAG-3′
NDV-Rev784 803-784 5′-TGYTGTSWRCARAAYTCRTG-3′
M1c 4175-4194 5′-TTCTCTAGCAGTGGGACAGC-3′
M2 4241-4264 5′-TGGAGCCAAACCCGCACCTGCGG-3′
F1 5095-5076 5′-CATCTTCCCAACTGCCACTG-3′
F2 5006-4988 5′-GGAGGATGTTGGCAGCATT-3′
NDV-For4359 4359-4382 5′-CCATTGCTAAATACAATCCTTTCA-3′
NDV-Rev4788 4788-4769 5′-GGGGCTTTYGCACACGCCTC-3′
NDV-For4988 4988-5007 5′-AATGCCGCCAACATCCTCCG-3′
NDV-Rev5261 5261-5241 5′-GTGCCTGGATAGTCAGCTGAG-3′
NDV-For5461 5461-5482 5′-GACYTTATCTGTAAGYACAACC-3′
NDV-Rev5731 5731-5711 5′-CAATTGGCAATAACTGAGCC-3′
NDV-For5918 5918-5940 5′-GTGACAGGCAAYCTTGATATATC-3′
NDV-Rev6204 6204-6185 5′-CTTGTAGTGGCTCTCATCTG-3′
NDV-For6369 6369-6388 5′-AGGCYTCACAACATCYGTTC-3′
NDV-Rev6598 6598-6579 5′-TYGATATGCCTRCGAGRTCG-3′
NDV-For13970 13970-13994 5′-GCAGTGGGATATATCACATCTGTGG-3′
NDV-For14492 14492-14511 5′-CAGCCYGTCCGTCCATTCTG-3′
NDV-Rev14739 14739-14720 5′-CTGAGACCCAGTATTGTGAC-3′
  1. aThe nucleotide position of primers corresponds with the whole genome nucleotide sequences of NDV strain U.S.(CA)/1083(Fontana)/72 (accession # AY562988); bTwenty nucleotide long of 5′-end of primer NDV-For1 is adapter sequence; cM1, M2, F1 and F2 primers are reported by Mase et al. [16].
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Virology Journal

ISSN: 1743-422X