Molecular epidemiology of Newcastle disease virus isolates from vaccinated commercial poultry farms in non-epidemic areas of Japan

Table 5 Primers used in this study

Primer name	Nucleotide position^a	Primer sequence
NDV-For1	1-15	5′-GGCCACGCGTCGACTAGTACACCAAACAGAGAATC-3′^b
NDV-For482	482-501	5′-TYTGAGGAGAGRGCACAGAG-3′
NDV-Rev784	803-784	5′-TGYTGTSWRCARAAYTCRTG-3′
M1^c	4175-4194	5′-TTCTCTAGCAGTGGGACAGC-3′
M2	4241-4264	5′-TGGAGCCAAACCCGCACCTGCGG-3′
F1	5095-5076	5′-CATCTTCCCAACTGCCACTG-3′
F2	5006-4988	5′-GGAGGATGTTGGCAGCATT-3′
NDV-For4359	4359-4382	5′-CCATTGCTAAATACAATCCTTTCA-3′
NDV-Rev4788	4788-4769	5′-GGGGCTTTYGCACACGCCTC-3′
NDV-For4988	4988-5007	5′-AATGCCGCCAACATCCTCCG-3′
NDV-Rev5261	5261-5241	5′-GTGCCTGGATAGTCAGCTGAG-3′
NDV-For5461	5461-5482	5′-GACYTTATCTGTAAGYACAACC-3′
NDV-Rev5731	5731-5711	5′-CAATTGGCAATAACTGAGCC-3′
NDV-For5918	5918-5940	5′-GTGACAGGCAAYCTTGATATATC-3′
NDV-Rev6204	6204-6185	5′-CTTGTAGTGGCTCTCATCTG-3′
NDV-For6369	6369-6388	5′-AGGCYTCACAACATCYGTTC-3′
NDV-Rev6598	6598-6579	5′-TYGATATGCCTRCGAGRTCG-3′
NDV-For13970	13970-13994	5′-GCAGTGGGATATATCACATCTGTGG-3′
NDV-For14492	14492-14511	5′-CAGCCYGTCCGTCCATTCTG-3′
NDV-Rev14739	14739-14720	5′-CTGAGACCCAGTATTGTGAC-3′

^aThe nucleotide position of primers corresponds with the whole genome nucleotide sequences of NDV strain U.S.(CA)/1083(Fontana)/72 (accession # AY562988); ^bTwenty nucleotide long of 5′-end of primer NDV-For1 is adapter sequence; ^cM1, M2, F1 and F2 primers are reported by Mase et al. [16].

ISSN: 1743-422X