Table 1 List of nucleotide sequence different between cloned pDEV-vac [GenBank: KF693236] and the published DEV vaccine sequence [GenBank: KF487736]
A. Changes in coding sequences | ||
|---|---|---|
Gene (according to KF487736) | Modification (nt position) | Effect |
UL36 | A to G (41 128) | Silent |
UL36 | A to C (43 596) | H to P |
UL23 | A to G (75 096) | I to V |
UL3 | C to T (110 795) | R to Q |
B. Changes in non-coding sequences | ||
Modification (nt position) | Comment | |
T to C (59 643) | Between UL30 and UL29 | |
Deletion 80 nt : GGGTTCCAAAGGTTTTACGGTA | Â | |
TTAGGGTTAGGGTTGGCAGGGTTCCAAAGGTTT | Â | |
TACGGTATTAGGGTTAGGGTTGGCA (119 773–119 852) | Between LORF2 and IR | |
Insertion: AA (129 421–129 422) | Between IR and US1 | |
T to G (143 005) | Between US7 and US8 | |
Insertion: TT (147 986–147 987) | Between US1 and TR | |
Deletion 80 nt: CCTAATACCGTAAAACCTTTGGAACC | Â | |
CTGCCAACCCTAACCCTAATACCGTAAAACCTTTGG | Â | |
AACCCTGCCAACCCTAAC (157 498–157 877) | Between TR and LORF11 | |
Insertion: G (158 090) | Between TR and LORF11 | |