Figure 6
Schematic drawing of the cloning procedure to introduce the pHA2 vector into the DEV-vac genome. (A) Diagram of the 158Â kb DEV-vac genome. Abbreviations: UL, unique long region; US, unique short region; TR, terminal repeat; IR, internal repeat. (B) The region of interest from UL19 to UL15A genes in DEV-vac genome. (C) The HA2 segment was inserted between UL15B and UL18 by transfection of transfer plasmid following infection with the DEV-vac genome. (D) The locations of the genes in pHA2 (including mini-F plasmid) are depicted by boxes. xgpt, xanthine-guanine phosphoribosyltransferase; cat, chloramphenicol resistance gene; gfp, green fluorescent protein gene; rep E, replication gene E; sopA, sopB, sopC, partitioning genes A, B, C; Pcmv, cytomegalovirus promoter. B, Bam H I; P, Pac I; H, Hin d III; E, Eco R I.